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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
06/12/2022 |
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Actualizado : |
06/12/2022 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
PEÑA-FLEITAS, M.T.; GRASSO, R.; GALLARDO, M.; PADILLA, F.M.; DE SOUZA, R.; RODRÍGUEZ , A.; THOMPSON, R.B. |
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Afiliación : |
M. TERESA PEÑA-FLEITAS, Department of Agronomy, Univ. of Almeria, La Cañada de San Urbano, Almeria, Spain; CIAIMBITAL Research Centre for Mediterranean Intensive Agrosystems and Agrifood Biotechnology, University of Almeria, La Cañada de San Urbano, 04120 Almeria, Spain; RAFAEL GILLSON GRASSO RODRIGUEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARISA GALLARDO, Department of Agronomy, Univ. of Almeria, La Cañada de San Urbano, Almeria, Spain; CIAIMBITAL Research Centre for Mediterranean Intensive Agrosystems and Agrifood Biotechnology, University of Almeria, La Cañada de San Urbano, 04120 Almeria, Spain; FRANCISCO M. PADILLA, Department of Agronomy, Univ. of Almeria, La Cañada de San Urbano, Almeria, Spain; CIAIMBITAL Research Centre for Mediterranean Intensive Agrosystems and Agrifood Biotechnology, University of Almeria, La Cañada de San Urbano, 04120 Almeria, Spain; ROMINA DE SOUZA, CENUR LN Departamento del Agua-Facultad de Agronomía, Universidad de la República, General Rivera 1350, Salto 50000, Uruguay; ALEJANDRA RODRÍGUEZ, University of Costa Rica, Sede Regional de Guanacaste, Guanacaste Province 50101, Costa Rica; RODNEY B. THOMPSON, Department of Agronomy, Univ. of Almeria, La Cañada de San Urbano, Almeria, Spain; CIAIMBITAL Research Centre for Mediterranean Intensive Agrosystems and Agrifood Biotechnology, University of Almeria, La Cañada de San Urbano, 04120 Almeria, Spain. |
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Título : |
Sample temperature affects measurement of nitrate with a rapid analysis ion selective electrode system used for N management of vegetable crops. |
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Fecha de publicación : |
2022 |
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Fuente / Imprenta : |
Agronomy, 2022, Volume 12, Issue 12, 3031. OPEN ACCESS. doi: https://doi.org/10.3390/agronomy12123031 |
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ISSN : |
2073-4395 |
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DOI : |
10.3390/agronomy12123031 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 27 October 2022; Revised 23 November 2022; Accepted 26 November 2022; Published: 30 November 2022. --
This article belongs to the Special Issue Growth and Nutrient Management of Vegetables (https://www.mdpi.com/journal/agronomy/special_issues/W0EN964704 ) --
Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). -- |
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Contenido : |
ABSTRACT.- The practical value of portable hand-held ion selective electrode sensors (ISE) for on-farm [NO3-] measurement to assist with crop N management of vegetable crops has been demonstrated in numerous previous studies. They provide rapid, in-situ measurement of the nitrate concentration ([NO3-]) in nutrient and soil solutions, and in petiole sap. Sample temperatures, for on-farm measurements, vary appreciably. This study evaluated the effects of sample temperature on [NO3-] measurement using two different models of a commonly used, commercially available, portable ISE meter. The temperatures (5, 10, 15, 20, and 25 °C) examined were in the range likely to be encountered in practical on-farm work. Aqueous solutions of 6, 12, and 18 mmol NO3-L-1 were prepared from KNO3, Ca(NO3)2 and NaNO3. [NO3-] was measured in three replicate samples of each of the three concentrations, made from each NO3-; compound, at each temperature. The results consistently and clearly demonstrated a strong negative linear relationship between temperature-induced errors and sample temperatures. The temperature-induced error was considerable for cooled samples, being +50% at 5 °C and +31% at 10 °C. At sample temperatures of 17-20 °C, the temperature effects were minimal. Above this range, the temperature effect caused underestimation. At 25 °C, the temperature-induced error was -24%. These results show that care must be taken to ensure that sample temperatures do not erroneously affect the measurement of [NO3-] with ISE meters. Particular care needs to be taken with both refrigerated and warmer samples.
© 2022 by the authors. MenosABSTRACT.- The practical value of portable hand-held ion selective electrode sensors (ISE) for on-farm [NO3-] measurement to assist with crop N management of vegetable crops has been demonstrated in numerous previous studies. They provide rapid, in-situ measurement of the nitrate concentration ([NO3-]) in nutrient and soil solutions, and in petiole sap. Sample temperatures, for on-farm measurements, vary appreciably. This study evaluated the effects of sample temperature on [NO3-] measurement using two different models of a commonly used, commercially available, portable ISE meter. The temperatures (5, 10, 15, 20, and 25 °C) examined were in the range likely to be encountered in practical on-farm work. Aqueous solutions of 6, 12, and 18 mmol NO3-L-1 were prepared from KNO3, Ca(NO3)2 and NaNO3. [NO3-] was measured in three replicate samples of each of the three concentrations, made from each NO3-; compound, at each temperature. The results consistently and clearly demonstrated a strong negative linear relationship between temperature-induced errors and sample temperatures. The temperature-induced error was considerable for cooled samples, being +50% at 5 °C and +31% at 10 °C. At sample temperatures of 17-20 °C, the temperature effects were minimal. Above this range, the temperature effect caused underestimation. At 25 °C, the temperature-induced error was -24%. These results show that care must be taken to ensure that sample temperatures do not erroneously affect the measurem... Presentar Todo |
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Palabras claves : |
Fertigation; Horiba; ISE; LAQUAtwin; N monitoring; Nutrient solution; Petiole sap; Quick tests; Soil solution. |
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Asunto categoría : |
F01 Cultivo |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/16909/1/agronomy-12-03031-v3.pdf
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Marc : |
LEADER 03153naa a2200337 a 4500
001 1063825
005 2022-12-06
008 2022 bl uuuu u00u1 u #d
022 $a2073-4395
024 7 $a10.3390/agronomy12123031$2DOI
100 1 $aPEÑA-FLEITAS, M.T.
245 $aSample temperature affects measurement of nitrate with a rapid analysis ion selective electrode system used for N management of vegetable crops.$h[electronic resource]
260 $c2022
500 $aArticle history: Received 27 October 2022; Revised 23 November 2022; Accepted 26 November 2022; Published: 30 November 2022. -- This article belongs to the Special Issue Growth and Nutrient Management of Vegetables (https://www.mdpi.com/journal/agronomy/special_issues/W0EN964704 ) -- Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). --
520 $aABSTRACT.- The practical value of portable hand-held ion selective electrode sensors (ISE) for on-farm [NO3-] measurement to assist with crop N management of vegetable crops has been demonstrated in numerous previous studies. They provide rapid, in-situ measurement of the nitrate concentration ([NO3-]) in nutrient and soil solutions, and in petiole sap. Sample temperatures, for on-farm measurements, vary appreciably. This study evaluated the effects of sample temperature on [NO3-] measurement using two different models of a commonly used, commercially available, portable ISE meter. The temperatures (5, 10, 15, 20, and 25 °C) examined were in the range likely to be encountered in practical on-farm work. Aqueous solutions of 6, 12, and 18 mmol NO3-L-1 were prepared from KNO3, Ca(NO3)2 and NaNO3. [NO3-] was measured in three replicate samples of each of the three concentrations, made from each NO3-; compound, at each temperature. The results consistently and clearly demonstrated a strong negative linear relationship between temperature-induced errors and sample temperatures. The temperature-induced error was considerable for cooled samples, being +50% at 5 °C and +31% at 10 °C. At sample temperatures of 17-20 °C, the temperature effects were minimal. Above this range, the temperature effect caused underestimation. At 25 °C, the temperature-induced error was -24%. These results show that care must be taken to ensure that sample temperatures do not erroneously affect the measurement of [NO3-] with ISE meters. Particular care needs to be taken with both refrigerated and warmer samples. © 2022 by the authors.
653 $aFertigation
653 $aHoriba
653 $aISE
653 $aLAQUAtwin
653 $aN monitoring
653 $aNutrient solution
653 $aPetiole sap
653 $aQuick tests
653 $aSoil solution
700 1 $aGRASSO, R.
700 1 $aGALLARDO, M.
700 1 $aPADILLA, F.M.
700 1 $aDE SOUZA, R.
700 1 $aRODRÍGUEZ , A.
700 1 $aTHOMPSON, R.B.
773 $tAgronomy, 2022, Volume 12, Issue 12, 3031. OPEN ACCESS. doi: https://doi.org/10.3390/agronomy12123031
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Registro original : |
INIA Las Brujas (LB) |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
11/08/2016 |
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Actualizado : |
12/03/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
ROCHA, C.M.L.; VELLICCE, G.R.; GARCÍA, M.G.; PARDO, E.M.; RACEDO, J.; PERERA, M.F.; DE LUCÍA, A.; BONNECARRERE, M.; GERMAN, S.; MARCELINO, F.; LEDESMA, F.; REZNIKOV, S.; PLOPER, D.L.; WELIN, B.; CASTAGNARO, A.P. |
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Afiliación : |
CARLA MARÍA LOURDES ROCHA, Estación Experimental Agroindustrial Obispo Colombres, Consejo Nacional de Investigaciones Científicas y Técnicas, Instituto de Tecnología Agroindustrial del Noroeste Argentino; GABRIEL RICARDO VELLICCE, Estación Experimental Agroindustrial Obispo Colombres, Consejo Nacional de Investigaciones Científicas y Técnicas, Instituto de Tecnología Agroindustrial del Noroeste Argentino; MARÍA GABRIELA GARCÍA, Estación Experimental Agroindustrial Obispo Colombres, Consejo Nacional de Investigaciones Científicas y Técnicas, Instituto de Tecnología Agroindustrial del Noroeste Argentino; ESTEBAN MARIANO PARDO, Estación Experimental Agroindustrial Obispo Colombres, Consejo Nacional de Investigaciones Científicas y Técnicas, Instituto de Tecnología Agroindustrial del Noroeste Argentino; JOSEFINA RACEDO, Estación Experimental Agroindustrial Obispo Colombres, Consejo Nacional de Investigaciones Científicas y Técnicas, Instituto de Tecnología Agroindustrial del Noroeste Argentino; MARÍA FRANCISCA PERERA, Estación Experimental Agroindustrial Obispo Colombres, Consejo Nacional de Investigaciones Científicas y Técnicas, Instituto de Tecnología Agroindustrial del Noroeste Argentino; ADRIÁN DE LUCÍA, INTA (Instituto Nacional de Tecnología Agropecuaria) - Cerro Azul; MARIA VICTORIA BONNECARRERE MARTINEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; SILVIA ELISA GERMAN FAEDO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FRANCISMAR MARCELINO, EMBRAPA Soja; FRANCISCO LEDESMA, Estación Experimental Agroindustrial Obispo Colombres, Consejo Nacional de Investigaciones Científicas y Técnicas, Instituto de Tecnología Agroindustrial del Noroeste Argentino; SEBASTIÁN REZNIKOV, Estación Experimental Agroindustrial Obispo Colombres, Consejo Nacional de Investigaciones Científicas y Técnicas, Instituto de Tecnología Agroindustrial del Noroeste Argentino; LEONARDO DANIEL PLOPER, Estación Experimental Agroindustrial Obispo Colombres, Consejo Nacional de Investigaciones Científicas y Técnicas, Instituto de Tecnología Agroindustrial del Noroeste Argentino; BJORN WELIN, Estación Experimental Agroindustrial Obispo Colombres, Consejo Nacional de Investigaciones Científicas y Técnicas, Instituto de Tecnología Agroindustrial del Noroeste Argentino; ATILIO PEDRO CASTAGNARO, Estación Experimental Agroindustrial Obispo Colombres, Consejo Nacional de Investigaciones Científicas y Técnicas, Instituto de Tecnología Agroindustrial del Noroeste Argentino. |
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Título : |
Use of AFLP markers to estimate molecular diversity of Phakopsora pachyrhizi. |
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Fecha de publicación : |
2015 |
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Fuente / Imprenta : |
Electronic Journal of Biotechnology, 2015, v. 18, no. 6, p. 439-444. OPEN ACCESS. |
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DOI : |
10.1016/j.ejbt.2015.06.007 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 24 April 2015. Accepted 23 September 2015. Available online 28 October 2015. |
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Contenido : |
ABSTRACT.
Background: Asian soybean rust (SBR) caused by Phakopsora pachyrhizi Syd. & Syd., is one of the main diseases affecting soybean and has been reported as one of the most economically important fungal pathogens worldwide. Knowledge of the genetic diversity of this fungus should be considered when developing resistance breeding strategies. We aimed to analyze the genetic diversity of P. pachyrhizi combining simple sampling with a powerful and reproducible molecular technique. Results: We employed Amplified Fragment Length Polymorphism (AFLP) technique for the amplification of P. pachyrhizi DNA extracted from naturally SBR-infected plants from 23 production fields. From a total of 1919
markers obtained, 77% were polymorphic. The high percentage of polymorphism and the Nei's genetic diversity coefficient (0.22) indicated high pathogen diversity. Analysis of molecular variance showed higher genetic variation within countries than among them. Temporal analysis showed a higher genetic variation within a year than between years. Cluster, phylogenetic and principal co-ordinate analysis showed that samples group by year of collection and then by country sampled. Conclusions: The study proposed combining a simple collection of urediniospore with a subsequent analysis by AFLP was useful to examine the molecular polymorphism of samples of P. pachyrhizi collected and might have a significant contribution to the knowledge of its genetic diversity. Also, AFLP analysis is an important and potent molecular tool for the study of genetic diversity and could be useful to carry out wider genetic diversity studies.
© 2015 Pontificia Universidad Católica de Valparaíso. Production and hosting by Elsevier B.V. All rights reserve MenosABSTRACT.
Background: Asian soybean rust (SBR) caused by Phakopsora pachyrhizi Syd. & Syd., is one of the main diseases affecting soybean and has been reported as one of the most economically important fungal pathogens worldwide. Knowledge of the genetic diversity of this fungus should be considered when developing resistance breeding strategies. We aimed to analyze the genetic diversity of P. pachyrhizi combining simple sampling with a powerful and reproducible molecular technique. Results: We employed Amplified Fragment Length Polymorphism (AFLP) technique for the amplification of P. pachyrhizi DNA extracted from naturally SBR-infected plants from 23 production fields. From a total of 1919
markers obtained, 77% were polymorphic. The high percentage of polymorphism and the Nei's genetic diversity coefficient (0.22) indicated high pathogen diversity. Analysis of molecular variance showed higher genetic variation within countries than among them. Temporal analysis showed a higher genetic variation within a year than between years. Cluster, phylogenetic and principal co-ordinate analysis showed that samples group by year of collection and then by country sampled. Conclusions: The study proposed combining a simple collection of urediniospore with a subsequent analysis by AFLP was useful to examine the molecular polymorphism of samples of P. pachyrhizi collected and might have a significant contribution to the knowledge of its genetic diversity. Also, AFLP analysis is an impor... Presentar Todo |
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Palabras claves : |
ASIAN SOYBEAN RUST; GENETIC VARIATION; GLYCINE MAX; MOLECULAR MARKERS. |
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Thesagro : |
MARCADORES MOLECULARES; SOJA; VARIACION GENETICA. |
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Asunto categoría : |
-- |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/5850/1/Rocha-C.M.-2015.-Electr.Jr.Biotech.-v18-p.439-444.pdf
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Marc : |
LEADER 02957naa a2200397 a 4500
001 1055241
005 2019-03-12
008 2015 bl uuuu u00u1 u #d
024 7 $a10.1016/j.ejbt.2015.06.007$2DOI
100 1 $aROCHA, C.M.L.
245 $aUse of AFLP markers to estimate molecular diversity of Phakopsora pachyrhizi.$h[electronic resource]
260 $c2015
500 $aArticle history: Received 24 April 2015. Accepted 23 September 2015. Available online 28 October 2015.
520 $aABSTRACT. Background: Asian soybean rust (SBR) caused by Phakopsora pachyrhizi Syd. & Syd., is one of the main diseases affecting soybean and has been reported as one of the most economically important fungal pathogens worldwide. Knowledge of the genetic diversity of this fungus should be considered when developing resistance breeding strategies. We aimed to analyze the genetic diversity of P. pachyrhizi combining simple sampling with a powerful and reproducible molecular technique. Results: We employed Amplified Fragment Length Polymorphism (AFLP) technique for the amplification of P. pachyrhizi DNA extracted from naturally SBR-infected plants from 23 production fields. From a total of 1919 markers obtained, 77% were polymorphic. The high percentage of polymorphism and the Nei's genetic diversity coefficient (0.22) indicated high pathogen diversity. Analysis of molecular variance showed higher genetic variation within countries than among them. Temporal analysis showed a higher genetic variation within a year than between years. Cluster, phylogenetic and principal co-ordinate analysis showed that samples group by year of collection and then by country sampled. Conclusions: The study proposed combining a simple collection of urediniospore with a subsequent analysis by AFLP was useful to examine the molecular polymorphism of samples of P. pachyrhizi collected and might have a significant contribution to the knowledge of its genetic diversity. Also, AFLP analysis is an important and potent molecular tool for the study of genetic diversity and could be useful to carry out wider genetic diversity studies. © 2015 Pontificia Universidad Católica de Valparaíso. Production and hosting by Elsevier B.V. All rights reserve
650 $aMARCADORES MOLECULARES
650 $aSOJA
650 $aVARIACION GENETICA
653 $aASIAN SOYBEAN RUST
653 $aGENETIC VARIATION
653 $aGLYCINE MAX
653 $aMOLECULAR MARKERS
700 1 $aVELLICCE, G.R.
700 1 $aGARCÍA, M.G.
700 1 $aPARDO, E.M.
700 1 $aRACEDO, J.
700 1 $aPERERA, M.F.
700 1 $aDE LUCÍA, A.
700 1 $aBONNECARRERE, M.
700 1 $aGERMAN, S.
700 1 $aMARCELINO, F.
700 1 $aLEDESMA, F.
700 1 $aREZNIKOV, S.
700 1 $aPLOPER, D.L.
700 1 $aWELIN, B.
700 1 $aCASTAGNARO, A.P.
773 $tElectronic Journal of Biotechnology, 2015$gv. 18, no. 6, p. 439-444. OPEN ACCESS.
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